标题：Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts作者：Deng, Songqiang; Wang, Chunzi; De Philippis, Roberto; Zhou, Xiangjun;Ye, Chaoran; Chen, Lanzhou

来源出版物：BIOLOGY AND FERTILITY OF SOILS 卷：52 期：5 页码：595-599 DOI: 10.1007/s00374-016-1107-7 出版年：JUL 2016

摘要：The quantitative PCR (qPCR) method was firstly used to measure moss abundance with moss chloroplast gene rps4 as compared to that with cyanobacterial 16S rRNA gene and fungal 25-28S rRNA gene in the early succession stage of biological soil crusts (BSCs). Four sites with three BSC types collected from Hobq Desert of China, representing cyanobacterial-, lichen-, and moss-dominated BSCs were investigated. The copies of the moss rps4 gene, cyanobacterial 16S rRNA gene, and fungal 25-28S rRNA gene, chlorophyll a content, and the community composition variated significantly. The moss rps4 gene copies attained a significant positive correlation with chlorophyll a content and showed a cross-validation with relative moss biomass.

入藏号：WOS:000377994900001

文献类型：Article

语种：English

作者关键词：Moss, Quantitative PCR, rps4, Biomass, Community composition

扩展关键词：16S RIBOSOMAL-RNA; MOJAVE DESERT; BACTERIAL; POLYSACCHARIDES; CYANOBACTERIA; COMMUNITIES; PHYLOGENY; DIVERSITY; PRIMERS; BIOMASS

通讯作者地址：Chen, LZ, Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Peoples R China.

电子邮件地址：

地址：

[Deng, Songqiang; Wang, Chunzi; Zhou, Xiangjun; Ye, Chaoran; Chen, Lanzhou] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Peoples R China.

[De Philippis, Roberto] Univ Florence, Dept Agrifood Prod & Environm Sci, Piazzale Cascine 24, I-50144 Florence, Italy.

研究方向：Agriculture

ISSN：0178-2762

eISSN: 1432-0789

影响因子：3.069