标题：Biodegradation of the Organophosphate Trichlorfon and Its Major Degradation Products by a Novel Aspergillus sydowii PA F-2作者：Tian, Jiang; Dong, Qiaofeng; Yu, Chenlei; Zhao, Ruixue; Wang, Jing;Chen, Lanzhou

来源出版物：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 卷：64 期：21 页码：4280-4287 DOI: 10.1021/acs.jafc.6b00909 出版年：JUN 1 2016

摘要：Trichlorfon (TCF) is an important organophosphate pesticide in agriculture. However, limited information is known about the biodegradation behaviors and kinetics of this pesticide. In this study, a newly isolated fungus (PA F-2) from pesticide-polluted soils was identified as Aspergillus sydowii on the basis of the sequencing of internal transcribed spacer rDNA. This fungus degraded TCF as sole carbon, sole phosphorus, and sole carbon-phosphorus sources in a mineral salt medium (MSM). Optimal TCF degradation conditions were determined through response surface methodology, and results also revealed that 75.31% of 100 mg/L TCF was metabolized within 7 days. The degradation of TCF was accelerated, and the mycelial dry weight of PA F-2 was remarkably increased in MSM supplemented with exogenous sucrose and yeast extract. Five TCF metabolic products were identified through gas chromatography-mass spectrometry. TCF could be initially hydrolyzed to dichlorvos and then be degraded through the cleavage of the P-C bond to produce dimethyl hydrogen phosphate and chloral hydrate. These two compounds were subsequently deoxidized to produce dimethyl phosphite and trichloroethanal. These results demonstrate the biodegradation pathways of TCF and promote the potential use of PA F-2 to bioremediate TCF-contaminated environments.

入藏号：WOS:000377150800012

文献类型：Article

语种：English

作者关键词：trichlorfon, biodegradation, Aspergillus sydowii, mycelial dry weight, biodegradation pathway

扩展关键词：IN-VITRO; PESTICIDES; IDENTIFICATION; FERMENTATION; OPTIMIZATION; KINETICS; STRAIN

通讯作者地址：Chen, LZ (reprint author), Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Hubei, Peoples R China.

电子邮件地址：chenlz@whu.edu.cn

地址：

[Tian, Jiang; Yu, Chenlei; Zhao, Ruixue; Wang, Jing; Chen, Lanzhou]Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Wuhan 430079, Hubei, Peoples R China.

[Dong, Qiaofeng] Inst Wuhan Modern Urban Agr Planning & Design, Wuhan 430072, Hubei, Peoples R China.

研究方向：Agriculture; Chemistry; Food Science & Technology

ISSN：0021-8561

eISSN: 1520-5118

影响因子：2.857