标题: Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y

作者: Li, H (Li, Hao); Long, M (Long, Min); Su, HY (Su, Huiyu); Tan, L (Tan, Lei); Shi, XW (Shi, Xiaowen); Du, YM (Du, Yumin); Luo, Y (Luo, Yan); Deng, HB (Deng, Hongbing)

来源出版物: CARBOHYDRATE POLYMERS 卷: 290 文献号: 119482 DOI: 10.1016/j.carbpol.2022.119482 出版年: AUG 15 2022

摘要: Immunoglobulin Y (IgY) proves advantageous to IgG in prophylaxis and diagnosis. Quantification of IgY is therefore becoming a topic of interest. Here, we demonstrate a piezoelectric biosensor with carboxymethyl chitosan (CMCS) as the immobilization matrix. Gelation and hydrophilic nature of CMCS are favored to form a crosslinked matrix for antibody immobilization, and a comparison was made between carboxymethyl cellulose (CMC) and CMCS to investigate the benefits of such substitution. Calibration from 500 ng/mL to 200 mu g/mL was established in buffer with the detection limit (LOD) down to 270 ng/mL, confirming its feasibility. As-prepared biosensor effectively prevents non-specific binding of bovine serum albumin (BSA) and lysozyme. Each real-time assay took 15 min including sensor regeneration, which can be further reduced to 4 min for signal readout only, ready for both repeated measurements after regeneration and disposable devices. Thus, as-prepared biosensor offers a rapid, label-free and cost-effective approach for IgY quantification.

作者关键词: Carboxymethyl chitosan; Carboxymethyl cellulose; Quartz crystal microbalance; Piezoelectric biosensor; Immunoassay

地址: [Li, Hao; Long, Min; Su, Huiyu; Shi, Xiaowen; Du, Yumin; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bio, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Tan, Lei; Luo, Yan] Shenzhen Inst Qual & Safety Inspection & Res, Shenzhen 518000, Peoples R China.

[Deng, Hongbing] Wuhan Univ, Shenzhen Res Inst, Shenzhen 518063, Peoples R China.

通讯作者地址: Deng, HB (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bio, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

Luo, Y (通讯作者)，Shenzhen Inst Qual & Safety Inspection & Res, Shenzhen 518000, Peoples R China.

Deng, HB (通讯作者)，Wuhan Univ, Shenzhen Res Inst, Shenzhen 518063, Peoples R China.

电子邮件地址: luoyan@mail.amr.sz.gov.cn; hbdeng@whu.edu.cn

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