标题: Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal olecular mechanisms for antibiotic removal

作者: Li, Z (Li, Zhuo); Li, SX (Li, Shuangxi); Li, TR (Li, Tianrui); Gao, XX (Gao, Xinxin); Zhu, LD (Zhu, Liandong)

来源出版物: ISCIENCE 卷: 25 期: 7 文献号: 104638 DOI: 10.1016/j.isci.2022.104638 出版年: JUL 15 2022

摘要: Microalgae-based technology is an effective and environmentally friendly method for antibiotics-contaminated wastewater treatment. To assess the tolerance and removal ability of Chlorella sorokiniana to ciprofloxacin (CIP), this study comprehensively revealed the responses of C. sorokiniana to CIP exposure and its degradation processes through physiological and transcriptomic analyses. Although the photosynthetic system was inhibited, the growth of C. sorokiniana was not negatively affected by CIP. Dissolved organic matter was analyzed and indicated that humic-like substances were released to alleviate the stress of CIP. In addition, the maximum removal of CIP was 83.3% under 20 mg L-1 CIP exposure. HPLC-MS/MS and RNA-Seq analyses suggested that CIP could be bioaccumulated and biodegraded by C. sorokiniana through the reactions of hydroxylation, demethylation, ring cleavage, oxidation, dehydrogenation, and decarboxylation with the help of intracellular oxidoreductases, especially cytochrome P450. Collectively, this research shows that C. sorokiniana have a great potential for removing CIP from wastewater.

地址: [Li, Zhuo; Li, Shuangxi; Li, Tianrui; Gao, Xinxin; Zhu, Liandong] Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm B, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

电子邮件地址: zhuoli2000@whu.edu.cn; ldzhu@whu.edu.cn

影响因子：6.107