标题: Mechanism of<i> A.</i><i> oleivorans</i> S4 treating soluble phosphorus deficiency and hydrocarbon contamination simultaneously

作者: Wang, PP (Wang, Panpan); Chen, CQ (Chen, Chaoqi); Liao, KJ (Liao, Kejun); Tao, Y (Tao, Yue); Fu, YJ (Fu, Yaojia); Chen, LZ (Chen, Lanzhou)

来源出版物: SCIENCE OF THE TOTAL ENVIRONMENT 卷: 949 文献号: 175215 DOI: 10.1016/j.scitotenv.2024.175215 Published Date: 2024 NOV 1

摘要: Both soluble phosphorus (P) deficiency and petroleum hydrocarbon contamination represent challenges in soil environments. While phosphate-solubilizing bacteria and hydrocarbon-degrading bacteria have been identified and employed in environmental bioremediation, the bacteria co-adapted to soluble P deficiency and hydrocarbon contamination has rarely been reported. This study explored the ability of Acinetobacter oleivorans S4 (A. oleivorans S4) to solubilize phosphate using n-hexadecane (H), glucose (G), and a mixed carbon source (HG) in tricalcium phosphate (TCP) medium. A. oleivorans S4 exhibited robust growth in H-TCP, releasing 31 mg L- 1 of soluble P. Conversely, A. oleivorans S4 barely grew in G-TCP, releasing 654 mg L- 1 of soluble P. In HG-TCP, biomass surpassed that in H-TCP, with phosphate release comparable to that in G-TCP. HPLC analysis revealed a small amount of TCA cycle acids in H-TCP and a large amount of gluconate in G-TCP and HG-TCP. Transcriptomic analysis showed elevated expression of genes associated with alkane degradation, P starvation, N utilization, and trehalose synthesis in H-TCP, revealing the molecular co-adaptation mechanism of A. oleivorans S4. Furthermore, the addition of glucose enhanced alkane degradation, P and N utilization, and reduced trehalose synthesis. It indicated that incomplete glucose metabolism may provide energy for other reactions, and the increase in soluble P mediated by gluconate may alleviate oxidative stress. Overall, A. oleivorans S4 proves promising for remediating soluble P-deficient and hydrocarbon-contaminated environments, and glucose stimulates its transformation into a super phosphate-solubilizing bacterium.

作者关键词: Petroleum hydrocarbons degradation; Phosphate solubilization; Incomplete glucose metabolism; A. oleivorans S4

地址: [Wang, Panpan; Chen, Chaoqi; Liao, Kejun; Tao, Yue; Fu, Yaojia; Chen, Lanzhou] Wuhan Univ, Hubei Res Ctr Environm Remediat Technol, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bio, Wuhan 430079, Peoples R China.

通讯作者地址: Chen, CQ; Chen, LZ (通讯作者)，Wuhan Univ, Hubei Res Ctr Environm Remediat Technol, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bio, Wuhan 430079, Peoples R China.

电子邮件地址: chenchaoqi@whu.edu.cn; chenlz@whu.edu.cn

影响因子：8.2