标题: Construction of a microalgal-fungal spore co-culture system for the treatment of wastewater containing Zn(II) and estrone: Pollutant removal and microbial biochemical reactions

作者: He, XM (He, Xiaoman); Liu, DY (Liu, Dongyang); Teng, Y (Teng, Yue); Wang, HZ (Wang, Hanzhi); Wu, QR (Wu, Qirui); Wang, W (Wang, Wei); Ren, JZ (Ren, Jingzheng); Zhu, LD (Zhu, Liandong)

来源出版物: JOURNAL OF ENVIRONMENTAL MANAGEMENT 卷: 375 文献号: 124221 DOI: 10.1016/j.jenvman.2025.124221 Published Date: 2025 FEB

摘要: The co-culture system of Chlorella sorokiniana and Aspergillus oryzae has demonstrated exceptional tolerance and efficiency in the removal of pollutants from swine manure. This study evaluates the ability of the co-culture system to remove Zn(II) and estrone, while assessing the impact of these pollutants on the system's overall functionality. Results indicated that co-cultivation achieved higher biomass accumulation, peaking at 0.88 g/L after 96 h. Increasing estrone exposure concentration reduced photosynthetic activity and chlorophyll content, whereas Zn(II) exposure initially enhanced and later inhibited chlorophyll synthesis. Co-cultivation secreted extracellular polymeric substances, including protein-like and humus-like substances, to alleviate environmental stress and form algal-fungal community. After 96 h of cultivation, the removal efficiencies reached 86.44% for 1.5 mg/L Zn(II) and 84.55% for 20 mg/L estrone. The Quantitative Structure Activity Relationship model revealed a reduction in the ecotoxicity of estrone intermediate products to varying degrees. Metabolomics analysis showed that exposure to estrone and Zn(II) significantly boosted the production of Gibberellic acid, Indole-3-acetic acid, and Zeatin riboside in Chlorella sorokiniana, while reducing Abscisic Acid levels. Furthermore, the exposure led to an increase in various metabolites in the Tricarboxylic acid cycle of the co-cultivation system, influencing the synthesis and metabolism of key biochemical components like carbohydrates, lipids, and proteins. These findings elucidate the biochemical responses of Chlorella sorokiniana-Aspergillus oryzae co-culture system to pollutants and provide insights into its potential application in the treatment of wastewater containing endocrine disrupting chemicals and heavy metals.

作者关键词: Chlorella sorokiniana; Aspergillus oryzae; Zinc; Estrone; Bioremediation; Microbial metabolomics

KeyWords Plus: NUTRIENT REMOVAL; CULTIVATION; ESTROGENS; ZINC

地址: [He, Xiaoman; Liu, Dongyang; Teng, Yue; Wang, Hanzhi; Wu, Qirui; Wang, Wei; Zhu, Liandong] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

[He, Xiaoman; Liu, Dongyang; Teng, Yue; Wang, Hanzhi; Wu, Qirui; Wang, Wei; Zhu, Liandong] Wuhan Univ, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Zhu, Liandong] Wuhan Univ, State Key Lab Water Resources & Hydropower Engn Sc, Wuhan 430072, Peoples R China.

[Ren, Jingzheng] Hong Kong Polytech Univ, Dept Ind & Syst Engn, Hung Hom, Kowloon, Hong Kong, Peoples R China.

[Liu, Dongyang] Minist Agr & Rural Affairs, Rural Energy & Environm Agcy, Beijing 100125, Peoples R China.

通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

Zhu, LD (通讯作者)，Wuhan Univ, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

电子邮件地址: ldzhu@whu.edu.cn

影响因子：8