标题: Copper Stimulates Microalgal-Fungal Consortia Metabolism and Enhances Nutrient Synergic Removal in Livestock Wastewater: Insights from Mathematical Modeling and Transcriptomic Evidence

作者: Li, SX (Li, Shuangxi); Wu, QR (Wu, Qirui); Chen, FN (Chen, Funing); Ma, QH (Ma, Qiaohong); Zhang, JS (Zhang, Jingshen); Zhu, LD (Zhu, Liandong)

来源出版物: ACS ES&T WATER  卷: 5  期: 6  页: 3457-3468  DOI: 10.1021/acsestwater.5c00276  Early Access Date: MAY 2025  Published Date: 2025 MAY 22  

引用的参考文献数: 47

摘要: Excessive residual Cu(II) substantially impedes the advanced treatment of livestock wastewater. The influence of Cu(II) on nutrient removal and microbial metabolism in microalgal-fungal consortia was investigated in this study via a combination of mathematical modeling and transcriptomic analysis. The increase of Cu(II) concentration from 0.1 to 0.5 mg/L promoted the maximum removal efficiency of ammonium nitrogen (NH4 +-N), total phosphorus (TP), and chemical oxygen demand (COD) by 83.62, 92.94, and 86.51%, respectively. The novel kinetic model revealed that optimal pollutant removal was achieved under 0.28 mg/L Cu(II). Transcriptomic analysis revealed that the improvement in NH4 +-N removal was primarily driven by the upregulation of key genes involved in assimilation, including the glutamine synthetase-glutamate synthase (GS-GOGAT) cycle and glutamate dehydrogenase (GDH) pathway. In addition, 0.2 mg/L Cu(II) enhanced microalgal photosynthesis while downregulating the activity of dehydrogenases involved in fungal carbohydrate metabolism, resulting in an increased polysaccharide content. However, Cu(II) downregulated amino acid biosynthesis, leading to an obvious decrease in protein content. Meanwhile, an increase in protein content was observed in dissolved organic matter (DOMs), as protein could complex with Cu(II), thus reducing its biotoxicity. This study comprehensively evaluated the impact of Cu(II) on nutrient removal by microalgal-fungal consortia, providing novel insights to advance livestock wastewater treatment strategies.

作者关键词: Cu(II); dissolved organic matters; gene expression; microalgal-fungal consortia; nutrients removal

KeyWords Plus: DISSOLVED ORGANIC-MATTER; FLUORESCENCE; MECHANISMS; TOXICITY; IONS

地址: [Li, Shuangxi; Wu, Qirui; Zhu, Liandong] Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Chen, Funing; Ma, Qiaohong] Minist Nat Resources, Tech Innovat Ctr Mine Geol Environm Restorat Engn, Nanning 530028, Peoples R China.

[Chen, Funing; Ma, Qiaohong] Nat Resources Ecol Restorat Ctr Guangxi Zhuang Aut, Nanning 530029, Peoples R China.

[Zhang, Jingshen] Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China.

[Zhang, Jingshen] Henan Acad Sci, Huang Huai Lab, Zhengzhou 450046, Peoples R China.

[Zhu, Liandong] Wuhan Univ, State Key Lab Water Resources Engn & Management, Wuhan 430072, Peoples R China.

通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resources & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

Zhu, LD (通讯作者)，Wuhan Univ, State Key Lab Water Resources Engn & Management, Wuhan 430072, Peoples R China.

电子邮件地址: ldzhu@whu.edu.cn

影响因子：4.3