标题: Enhanced lead-zinc tailing remediation by inoculating cyanobacteria to induce biological soil crusts

作者: Hou, LH (Hou, Lianghui); Li, DY (Li, Dongyang); Wan, SH (Wan, Shuhai); Liao, KJ (Liao, Kejun); Tao, Y (Tao, Yue); Chen, LZ (Chen, Lanzhou)

来源出版物: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS  卷: 90  文献号: 104103  DOI: 10.1016/j.algal.2025.104103  Published Date: 2025 AUG  

摘要: The high concentration of heavy metal (HM) and nutrient deficiency in tailings result in prolonged and challenging natural remediation. As the initial of ecological succession, biological soil crusts (BSCs) play a crucial role in tailing bioremediation. However, research in this area is limited. In this study, a significant increase in chlorophyll a content, total DNA content, and species abundance was discovered by inoculating indigenous microalgae on the surface of lead-zinc tailing sand. After 60 days of inoculation, the soil-saturated moisture content and cation exchange capacity increased significantly by 24.4 % and 11.7 %, and soil soluble salt content decreased significantly by 52.9 %. Cyanobacteria reduced the available HM in the tailings through biosorption and responded to the oxidative stress by increasing the activity of the antioxidant enzyme system. After the experimental cycle ended, soil DTPA-Pb/Zn content decreased significantly by 12.5 % and 14.3 %, while the soil soluble protein, malondialdehyde content, and activities of antioxidant enzymes (SOD, CAT, POD and TPx) showed a significant increase. The maximum photochemical efficiency (Fv/Fm) and electron transport yield (Eto/CSo) of PS II significantly decreased (0.6 and 129.1 times), and the QA reduction energy (TRo/RC) significantly increased (0.3 times) compared with microalgal cells. The soil concentration of C, N, and P nutrients, as well as soil enzyme activities, significantly increased in induced BSCs. The expression levels of soil N and P-cycling genes also significantly increased. Results indicate that the induced BSCs exhibit strong adaptability and excellent restorability to the lead-zinc tailing area. Our study provides a novel approach for the ecological and rapid restoration of HM tailing soil.

作者关键词: Antioxidant system; Biological soil crusts; N/P-cycling genes; Photosystem II; Tailing remediation

KeyWords Plus: CADMIUM; WATER; NITROGEN; STRESS

地址: [Hou, Lianghui; Liao, Kejun; Tao, Yue; Chen, Lanzhou] Wuhan Univ, Sch Resource & Environm Sci, Wuhan, Hubei, Peoples R China.

[Li, Dongyang; Wan, Shuhai] Zhejiang Acad Surveying & Mapping Sci & Technol, Hangzhou, Peoples R China.

通讯作者地址: Chen, LZ (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Wuhan 430079, Peoples R China.

电子邮件地址: chenlz@whu.edu.cn

影响因子：4.5